murine vsmc cell line movas  (ATCC)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: A Lindera obtusiloba Extract Blocks Calcium-/Phosphate-Induced Transdifferentiation and Calcification of Vascular Smooth Muscle Cells and Interferes with Matrix Metalloproteinase-2 and Metalloproteinase-9 and NF- κ B

doi: 10.1155/2015/679238

Figure Lengend Snippet: Effects of LOE on the viability of VSMC. Cell viability of murine (a) and human (b) VSMC was quantified fluorometrically after 24–48 h treatment with different concentrations of LOE by intracellular esterase-mediated conversion of calcein AM and was calculated in relation to vehicle treated cells (100%). Shown are means ± SD ( n = 3; ns not significant).

Article Snippet: The murine VSMC cell lines (MOVAS-1; ATCC CRL-2797 and human aortic VSMC) (Life Technologies, Karlsruhe, Germany) were cultured in a humidified atmosphere at 37°C and 5% CO 2 .

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: A Lindera obtusiloba Extract Blocks Calcium-/Phosphate-Induced Transdifferentiation and Calcification of Vascular Smooth Muscle Cells and Interferes with Matrix Metalloproteinase-2 and Metalloproteinase-9 and NF- κ B

doi: 10.1155/2015/679238

Figure Lengend Snippet: Effects of LOE on Ca/P-induced mRNA expressions of osterix, osteocalcin, α -SMA, and SM22 α . The mRNA expressions of osterix and osteocalcin ( n = 3) (a) and of α -SMA and SM22 α ( n = 4) (b) in murine VSMC were determined by RT-PCR. The expression pattern after 24 h of stimulation is shown (means ± SD; ns not significant; ∗ P ≤ 0.05; ∗∗ P ≤ 0.01; ∗∗∗ P = 0.001).

Article Snippet: The murine VSMC cell lines (MOVAS-1; ATCC CRL-2797 and human aortic VSMC) (Life Technologies, Karlsruhe, Germany) were cultured in a humidified atmosphere at 37°C and 5% CO 2 .

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: A Lindera obtusiloba Extract Blocks Calcium-/Phosphate-Induced Transdifferentiation and Calcification of Vascular Smooth Muscle Cells and Interferes with Matrix Metalloproteinase-2 and Metalloproteinase-9 and NF- κ B

doi: 10.1155/2015/679238

Figure Lengend Snippet: Effects of LOE on Ca/P-induced mRNA expression of MMP-2 and MMP-9 and gelatinolytic activities in VSMC supernatants. (a) Shown are the treatment dependent mRNA expression levels of MMP-2 and MMP-9 in murine VSMC after 24–48 h ( n = 3). (b) Shown is a representative result of one measurement of gelatinolytic activities in VSMC supernatants by cleavage of DQ-gelatin and (c) the corresponding means ± SD of the respective endpoints of three independent measurements ( ns not significant; ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001).

Article Snippet: The murine VSMC cell lines (MOVAS-1; ATCC CRL-2797 and human aortic VSMC) (Life Technologies, Karlsruhe, Germany) were cultured in a humidified atmosphere at 37°C and 5% CO 2 .

Techniques: Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: A Lindera obtusiloba Extract Blocks Calcium-/Phosphate-Induced Transdifferentiation and Calcification of Vascular Smooth Muscle Cells and Interferes with Matrix Metalloproteinase-2 and Metalloproteinase-9 and NF- κ B

doi: 10.1155/2015/679238

Figure Lengend Snippet: Effects of LOE on Ca/P-induced activation of NF- κ B in VSMC. Treatment dependent changes in luciferase activity as an indicator for activation or inhibition of NF- κ B were detected in murine VSMC after 24 h. Shown are means ± SD of three independent measurements. ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001.

Article Snippet: The murine VSMC cell lines (MOVAS-1; ATCC CRL-2797 and human aortic VSMC) (Life Technologies, Karlsruhe, Germany) were cultured in a humidified atmosphere at 37°C and 5% CO 2 .

Techniques: Activation Assay, Luciferase, Activity Assay, Inhibition